FastQCFastQC Report
Mon 27 Feb 2023
SRR3126850.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126850.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460978
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191150.7767237252832004No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA49300.20032686192237395No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT46500.18894927138722897No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45960.18675502178402245No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG29930.1216183159703175TruSeq Adapter, Index 14 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC27860.11320702582469244Illumina PCR Primer Index 3 (95% over 23bp)
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT27280.110850239213841No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG26290.10682744827462903TruSeq Adapter, Index 14 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67400.083.8260651
ATAGCGG5100.083.225131
CGTAGGG6250.082.960912
ACGGGAT6250.074.664824
AGGGTAC11100.074.511785
GAGGGTA13150.073.8418054
AGGGCAT23000.073.7589345
AGTAAGG12900.073.483281
AAGGGTA17800.072.823834
AGGGATG30950.071.970245
CGAAGGG21150.071.763952
AGTAGGG43600.071.678332
GTAGGGC9900.070.943393
ATAGGGC12700.070.890893
AGAGGGC32350.070.6688843
AGGGTAG10450.070.602265
AAGGGAC21100.070.593614
TAAGGGA19500.070.584463
AGGGCAC16600.069.923655
TAGGGCA13200.069.2769244