FastQCFastQC Report
Mon 27 Feb 2023
SRR3126849.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126849.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2471077
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182880.7400821585082132No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA47220.1910907673051062No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT46390.18773190798991696No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45760.18518241236513472No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG31820.12876976314376282TruSeq Adapter, Index 14 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC28420.11501058040684284Illumina PCR Primer Index 3 (95% over 23bp)
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT27420.11096376195480755No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG26960.10910222546687133TruSeq Adapter, Index 14 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65150.082.718481
CGTAGGG5600.079.7769852
ATAGCGG5050.079.220771
AGGGTAC10400.077.272255
AAGGGTA18000.076.4990164
ATAGGGC15100.076.251763
AGGGTAT14650.074.744465
ACGGGAT7100.074.1345144
AGGGATG30000.073.157085
AGGGCAT22550.073.151185
TAGGGTA8450.072.301514
GAGGGTA12500.072.185834
CGAAGGG24300.072.18462
TAGGGCA13750.071.775684
GAATAGG15450.071.589761
AGTAGGG44300.070.592732
GTAGGGT9550.070.370953
AGGGCAC16300.070.3498155
AGTAAGG13150.070.152291
TAAGGGA21200.069.828933