Basic Statistics
Measure | Value |
---|---|
Filename | SRR3126849.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2471077 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 18288 | 0.7400821585082132 | No Hit |
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA | 4722 | 0.1910907673051062 | No Hit |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 4639 | 0.18773190798991696 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 4576 | 0.18518241236513472 | No Hit |
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG | 3182 | 0.12876976314376282 | TruSeq Adapter, Index 14 (95% over 24bp) |
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC | 2842 | 0.11501058040684284 | Illumina PCR Primer Index 3 (95% over 23bp) |
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT | 2742 | 0.11096376195480755 | No Hit |
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG | 2696 | 0.10910222546687133 | TruSeq Adapter, Index 14 (95% over 24bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 6515 | 0.0 | 82.71848 | 1 |
CGTAGGG | 560 | 0.0 | 79.776985 | 2 |
ATAGCGG | 505 | 0.0 | 79.22077 | 1 |
AGGGTAC | 1040 | 0.0 | 77.27225 | 5 |
AAGGGTA | 1800 | 0.0 | 76.499016 | 4 |
ATAGGGC | 1510 | 0.0 | 76.25176 | 3 |
AGGGTAT | 1465 | 0.0 | 74.74446 | 5 |
ACGGGAT | 710 | 0.0 | 74.134514 | 4 |
AGGGATG | 3000 | 0.0 | 73.15708 | 5 |
AGGGCAT | 2255 | 0.0 | 73.15118 | 5 |
TAGGGTA | 845 | 0.0 | 72.30151 | 4 |
GAGGGTA | 1250 | 0.0 | 72.18583 | 4 |
CGAAGGG | 2430 | 0.0 | 72.1846 | 2 |
TAGGGCA | 1375 | 0.0 | 71.77568 | 4 |
GAATAGG | 1545 | 0.0 | 71.58976 | 1 |
AGTAGGG | 4430 | 0.0 | 70.59273 | 2 |
GTAGGGT | 955 | 0.0 | 70.37095 | 3 |
AGGGCAC | 1630 | 0.0 | 70.349815 | 5 |
AGTAAGG | 1315 | 0.0 | 70.15229 | 1 |
TAAGGGA | 2120 | 0.0 | 69.82893 | 3 |