FastQCFastQC Report
Mon 27 Feb 2023
SRR3126847.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126847.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225321
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148250.6661960229557893No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT61150.2747918165514099No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA40730.18302977413146237No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24580.11045597466612682No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT23790.10690592503283795No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57350.083.7029951
AGGGCAT19550.075.9593355
TAGCGGG11950.073.352882
AGTAGGG38100.073.22662
TAGGGCA13550.072.689114
CGTAGGG5650.072.5676352
ATAGGGC14950.072.501833
AGGGATG23300.072.205125
ATAGCGG4800.071.7147141
TAGGGCG4850.070.9322054
TAAGGGA19700.069.852013
GTAGGGT7450.068.949733
AGGGCAA21550.068.691685
GTAGGGC11600.068.657983
GAGGGAT25850.068.000274
GAATAGG12450.067.7969741
GTAAGGG36300.067.380292
GAAGGGC30550.067.257023
TAGAGGG41850.067.228132
AGACGGG14600.067.140192
Produced by FastQC (version 0.11.5)