FastQCFastQC Report
Mon 27 Feb 2023
SRR3126846.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126846.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221649
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141780.6381746171424919No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT60160.2707898502418699No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39780.17905618754357686No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24690.11113366692938444No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT24220.10901812122436982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54800.083.144241
GAATAGG11850.075.072491
TAGGGCG4950.075.0039754
CGTAGGG5650.074.9100342
ATAGGGC13800.074.240323
AGTAGGG39550.073.4831852
GTAGGGC11450.073.059533
TAGCGGG10550.072.657612
TAGGGCA12600.071.986194
AGGGATG26000.071.5787665
TAGGGTA6100.070.879464
AGGGCAT20500.070.379625
AGTAAGG13900.070.095951
GGGCGAT11650.069.788296
ATAGGGA19800.069.307473
CGAAGGG19550.068.0746842
GTAGGGT8100.067.883353
TAAGAGG16350.067.365171
TAGACGG3650.067.057571
AGAGGGC28550.066.831673
Produced by FastQC (version 0.11.5)