FastQCFastQC Report
Mon 27 Feb 2023
SRR3126845.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126845.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2325356
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147820.6356876108432429No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT61280.26352954128314116No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41910.18023046793695244No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT25540.10983264497995146No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24910.10712338239822203No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53450.082.5787351
CGTAGGG5300.076.254532
TAGCGGG11750.075.190522
AGGGCAT19700.074.427175
AGTAAGG13250.074.223861
AGTAGGG38550.072.7768252
ATAGGGC15500.072.1586463
TAGGGCG5500.071.772774
GTAGGGT8300.071.340413
TAAGGGA18150.070.944223
GAATAGG14750.070.184911
AGGGTAC10250.070.147255
GTAAGGG38300.070.061662
ATAGCGG5450.069.9361341
TAGGGCA12700.069.9360664
AAGGGAC21500.069.5075154
AAGGGTA14350.069.426794
GGTAAGG16500.068.729981
GGGCGAT11650.068.575056
ACGGGAT4950.067.405654
Produced by FastQC (version 0.11.5)