FastQCFastQC Report
Mon 27 Feb 2023
SRR3126844.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126844.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246655
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168670.7507605751661915No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT56410.25108438990410187No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34290.15262690533259446No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT31400.13976333704996985No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27220.12115789918790379No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59400.083.9024051
CGTAGGG7600.081.293132
AGGGATG30650.077.4262855
ATAGAGG13750.072.07981
AGTAGGG37600.071.872532
AGGGCAT17800.071.2805945
TAGAGGG40700.070.453942
TGTAGGG17150.070.399892
ATAGGGC12600.070.367773
TATAGGG18150.069.639282
GGTAAGG14550.069.0897451
AGAGGGC27750.069.000073
TAGGGCA12100.068.988134
ATAGGGA21500.068.658193
CGAAGGG23800.068.5638662
GTAGGGC10100.068.173873
TAAGGGA19950.068.082583
AGTAAGG14050.067.853461
AAGAGGG77750.067.57422
TAAGAGG15800.067.506921
Produced by FastQC (version 0.11.5)