FastQCFastQC Report
Mon 27 Feb 2023
SRR3126843.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126843.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2253950
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161830.7179839836731072No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT56290.24973934648062293No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33680.1494265622573704No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT30910.13713702610971848No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28210.12515805585749462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57500.084.2016751
ACGGGTA3200.077.83914
CGTAGGG7600.076.088262
AGGGATG29900.074.032375
TGTAGGG16400.071.094162
TAGGGCA13250.070.229774
AGTAGGG37700.069.834942
TAGCGGG10850.069.762532
TAGGGTC6150.069.5405654
GGTAAGG13500.069.357351
GTAGGGT7950.069.165683
ACGGGAT5450.068.98674
GGGCGAT9900.068.8342746
AGGGCAT17700.068.7699055
TAGGGCG4450.068.647584
TAAGGGA17600.068.092523
AAGAGGG77950.067.429792
AGTAAGG15650.067.345241
TATAGGG18850.067.3408362
ATAGCGG5450.067.339721
Produced by FastQC (version 0.11.5)