FastQCFastQC Report
Mon 27 Feb 2023
SRR3126842.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126842.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2354273
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168820.7170791152937659No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT57510.24427923184779332No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34650.14717919289734027No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT31900.13549830457215453No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27990.11889020517161773No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG23840.10126268278997382No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60050.083.4576341
CGTAGGG6200.078.073342
AGGGATG31850.076.284865
TAGGGCA13250.073.774234
TAGGGTA7400.072.3986364
AGTAAGG16350.072.241281
ATAGAGG16000.071.468671
TATAGGG19650.070.7923662
GAATAGG14050.070.670081
AGTAGGG38150.070.3394242
AGGGCAT20650.069.412395
GAGGGAT25550.069.159874
TAGAGGG43100.068.803312
CGAAGGG22600.068.4139562
TGTAGGG18800.068.2436142
AAGAGGG84000.068.143622
GGTAAGG15000.068.076621
TAAGGGA21450.067.919053
AGGGACT40000.067.9086465
GTAGGGT8950.067.736683
Produced by FastQC (version 0.11.5)