FastQCFastQC Report
Mon 27 Feb 2023
SRR3126841.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126841.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2167746
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213940.9869237447560738No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42540.1962407034772524No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24090.11112925591835944No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA23170.1068852162568862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79550.085.942471
ACGGGAT5100.074.8777164
AGGGATG31600.072.868875
AGGGTAC8750.071.96645
AGTAAGG14950.071.957041
TAGAGGG42550.071.5765462
ATAGGGC14050.071.472843
GACCGAT1250.071.4293448
AGTAGGG37300.071.413022
GTAGGGC11650.071.2236943
GTACGGG4450.069.923262
AGAGGGC31100.069.58093
ATAGGGA20900.068.800453
CGTAGGG6400.068.507922
AGGGCAT19400.068.3093955
TAGCGGG11250.067.889142
TAAGGGA19600.067.590863
AAGGGTA14100.067.541394
TAGGGCG3700.067.5323644
TGTAGGG15450.067.437522
Produced by FastQC (version 0.11.5)