FastQCFastQC Report
Mon 27 Feb 2023
SRR3126840.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126840.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166006
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206130.9516594136858347No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT43480.20073813276602187No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25120.11597382463391145No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA22680.10470885122201877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74250.086.313671
CGTAGGG6400.072.734942
AGGGATG30550.071.994935
TAAGAGG16350.071.372821
GTAGGGC10550.071.27463
TAGGGCA11750.070.395044
AGTAGGG36550.070.2413942
TAGGGCG3900.069.892524
GAATAGG14700.069.4610441
AGGGCAT16050.069.1041645
GGGCGAT9050.069.0669566
AGGGCGA10500.068.480895
AGAGGGC30750.068.317133
AAGGGTA16900.068.1312944
AGGGTAC9900.067.884115
ATAGGGA19250.067.62643
TAGGGTC5650.067.3757864
TAGCGGG9550.066.961232
ACGGGAT5150.066.616664
CGAAGGG22100.066.594742
Produced by FastQC (version 0.11.5)