FastQCFastQC Report
Mon 27 Feb 2023
SRR3126839.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126839.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2279050
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214630.9417520458085606No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT43370.19029858932449922No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25490.11184484763388253No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA23630.10368355235734189No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79200.085.5246051
CGTAGGG8150.073.8077852
AGGGATG30950.073.3392265
AGTAAGG16900.071.581681
TGTAGGG15150.070.7248462
TAGGGCA13150.070.4028554
ATAGCGG4950.070.3690951
ATAGGGA18850.070.305223
AGGGTAC8850.070.093945
AGTAGGG35350.070.0601352
GTAGGGC12650.069.842073
GAATAGG15400.068.467231
CGAAGGG23250.068.1171952
AGGGCAT19700.067.748855
GGTAAGG13300.067.5985261
TAGAGGG45650.067.532512
ATAAGGG32950.067.4614262
AAGGGAC20250.066.837044
ACGGGAT5000.066.732624
GACCGAT1200.066.5759668
Produced by FastQC (version 0.11.5)