FastQCFastQC Report
Mon 27 Feb 2023
SRR3126836.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126836.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774225
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT482962.7220899265876652No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT54150.3052036804802097No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT52580.2963547464385859No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT44160.2488974059096225No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT43350.24433203229579115No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC28420.16018261494455324Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG27600.15556087869351407No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTC24920.14045569192182503No Hit
CGTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC23670.13341036227085065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT149550.088.620591
ATAGGGC11900.074.244183
AGGGATG19100.072.829925
GTAGGGC8400.072.170873
GGGCGAT7050.071.992326
TAGGGCA10050.071.54464
AGTAGGG25650.071.087932
ATAGCGG3900.069.9925461
GTACGGG4250.069.6631552
AGGGCAT12450.069.0769655
GAAGGGC23950.068.481223
ATAGAGG11450.068.232461
CTAACGG1450.068.161591
AGTAAGG11400.067.706041
GAATAGG11000.067.600951
AATAGGG22850.066.841882
AGAGGGC26900.066.7363743
GTAGGGT6650.066.4293
ATAGGGA14150.066.091893
AGGGAAT19850.066.0534065