FastQCFastQC Report
Mon 27 Feb 2023
SRR3126835.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126835.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305620
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236771.0269255124435077No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT63080.2735923525993008No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31180.13523477416052948No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG26570.11524015232345311No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85500.085.687551
ATAGGGC15050.072.987473
ATAGAGG15450.072.97631
CGTAGGG6550.072.695742
ACGGGTA2150.072.360974
AGTAAGG15200.071.693671
AGGGCAT18250.071.326295
TAGGGTA5900.071.115934
AGGGATG29700.069.935645
ACGGGAT5350.068.7336964
TAGCGGG13800.068.325022
CGAAGGG25650.068.189172
TAAGGGA20150.068.0842743
AGTAGGG39750.068.077512
TAGGGCA14950.067.484094
TAGGGCG3850.067.348954
TAGAGGG40700.067.0676352
GTACGGG5700.066.9944842
GAATAGG14450.066.9265441
ATAGCGG5100.066.6000751
Produced by FastQC (version 0.11.5)