FastQCFastQC Report
Mon 27 Feb 2023
SRR3126834.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126834.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309833
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225650.9769104519677397No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT62640.2711884365666262No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA30340.13135148731531673No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG27410.1186665875844704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84250.085.068211
CGTAGGG6450.078.734052
ATAGCGG6200.075.141661
TAGGGCG4200.074.971874
ATAGGGC14650.072.821753
ACGGGAT5200.072.3035354
ACGGGTA2650.070.939314
AGGGCAT20500.069.693555
AGTAGGG39650.069.49492
GTACGGG6500.069.4474642
AGGGATG31000.068.97995
TAAGGGA22900.068.546273
AAGGGCG11950.068.4312064
CGAAGGG25300.068.395232
AACGAGG9600.068.136661
ATAGAGG15150.068.025031
GAATAGG14200.067.605051
GACCGAT1600.067.558618
AGTAAGG17600.067.37911
TAGCGGG13200.067.326562
Produced by FastQC (version 0.11.5)