FastQCFastQC Report
Mon 27 Feb 2023
SRR3126833.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126833.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2421638
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236430.9763226378178737No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT64220.26519240282816836No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA30670.12664981306041614No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG27910.11525256871588568No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90000.084.760231
GACCGAT1900.079.152538
TAGGGCG4050.075.426984
ACGGGAT5250.074.299714
ATAGCGG5150.073.102861
ATAGGGC15600.072.604043
CGTAGGG6300.072.360172
AGGGATG30750.070.457015
TAGGGCA13700.070.323694
AGTAGGG39400.070.256652
GGTAAGG13450.069.977651
AGTAAGG16800.069.74961
ATAGGGT11500.069.473553
AGGGCAC17250.069.473555
ATAGAGG16400.069.442151
TAAGGGA21750.069.144743
TAGCGGG12650.068.7305152
TAACGGG8500.067.454252
AGAAGGG83700.067.09822
GAATAGG15950.066.9756241
Produced by FastQC (version 0.11.5)