FastQCFastQC Report
Mon 27 Feb 2023
SRR3126832.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126832.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2580549
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260071.0078088034755395No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40730.1578346313129493No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36700.1422177993907498No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33570.1300885974263616No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC27220.10548143050180409No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94550.086.2891851
ACGGGTA2700.082.048294
TAGGGCG3750.077.928414
AGGGATG42650.075.586235
TAGCGGG13000.075.416072
ATAGCGG5500.074.5982061
CGAAGGG21850.073.129422
CGTAGGG9450.072.8223952
ACGGGAT8300.072.688774
TAGGGCA14750.071.5799564
AGAGGGC37000.070.955963
GTAGGGC15800.070.701193
AGTAGGG39450.070.6129462
TAACGGG9200.070.190222
ATAGGGC15600.070.09693
AGTAAGG15900.069.998181
TACGGGT1700.069.314893
AAGGGTA16000.068.9336554
GTACGGG6300.068.832122
TGAAGGG44450.068.820342
Produced by FastQC (version 0.11.5)