FastQCFastQC Report
Mon 27 Feb 2023
SRR3126831.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126831.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583685
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246900.9556118489676567No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39150.15152775976947655No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36670.14192906643031175No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33610.1300855173908584No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC26650.10314724898739591No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89300.086.7946851
ACGGGTA2650.078.034364
ATAGCGG4750.077.2773061
TAGGGCG4450.076.041664
CGAAGGG21450.075.202412
CGTAGGG7650.073.770842
TAAGGGA24750.073.1079563
AGGGATG40150.072.80885
TAGCGGG11950.072.412722
AGGGCAT21350.072.202975
AATGCGG5300.071.921721
ACGGGAT9250.071.6401444
ATAGGGC17850.071.089363
AGAGGGC37650.070.528163
GTACGGG5950.070.345772
TAGGGCA15150.070.109184
AGTAAGG17200.069.2218251
GTAGGGC13550.068.6759953
AGTAGGG41050.068.1661452
GGTAAGG18050.067.787111
Produced by FastQC (version 0.11.5)