FastQCFastQC Report
Mon 27 Feb 2023
SRR3126830.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126830.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742852
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261710.9541528307032242No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39850.1452867307459535No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36860.13438566863979537No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34820.12694815469445672No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG28200.10281269277379895No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC27970.10197414953486371No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG27870.10160956551793536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT103550.086.084611
ACGGGTA3000.073.6258854
TAGGGCG5650.073.196134
CGAAGGG24150.072.388872
ATAGGGC17700.071.4208453
AGGGATG40900.071.124835
TAGGGCA15300.070.95364
ACGGGAT9600.070.492874
AGGGCAT23150.070.2391055
AGAGGGC41200.070.149383
GGTAAGG18150.070.0136641
AGGGTAC11450.069.77465
CGTAGGG9500.069.7495652
GTAGGGC13350.069.347533
AGGGAAT36200.068.9350055
GTACGGG6150.068.772272
AGTACGG3300.068.4577941
AGTAGGG42850.067.9965442
AAGGGCA37200.067.8399054
ATAGCGG5300.067.489051
Produced by FastQC (version 0.11.5)