FastQCFastQC Report
Mon 27 Feb 2023
SRR3126829.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126829.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983551
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160680.810062357862238No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT49920.25166985875331666No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA38370.19344095513551202No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24900.12553244156565674No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT20780.10476161187688142No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58850.085.06631
ACGGGAT5350.078.42444
CGTAGGG6900.074.473782
GTACGGG4650.074.010942
AGGGATG25250.073.513855
CGAAGGG19550.073.308242
AGTAAGG11850.072.4672241
TAAGGGA16550.072.3520363
AAGGGTA12650.070.8073044
AGTAGGG35500.069.719932
AAGAGGG69750.069.212062
GGGCGAT8600.068.850266
ATAGGGC11000.068.571293
TGTAGGG14250.068.4827962
AGGGCAT16500.068.35355
TAGAGGG35550.068.030522
AGACGGG13600.067.249462
AGGGTAC7600.066.779575
AGAGGGC24800.066.532123
AAGGGCA31050.066.50094
Produced by FastQC (version 0.11.5)