FastQCFastQC Report
Mon 27 Feb 2023
SRR3126828.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126828.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980986
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152540.7700205857083291No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT49130.2480078102520664No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35890.18117240606445478No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24560.12397866516976898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT52750.082.968881
GTAGGGC9450.072.6114963
CGAAGGG17900.072.5200962
AGTAGGG35550.072.103982
TAGGGCG4350.071.308124
ACGGGAT5150.071.182254
AGGGATG27400.069.811695
ATAGGGC11400.069.673263
GTAAGGG30650.069.206742
GTACGGG5000.068.668122
ATGAGGG33950.068.15972
TAAGGGA17300.067.373623
GGTAAGG11700.067.171551
GAATAGG12000.067.060931
AAGAGGG69550.067.016052
TGTAGGG14250.066.671292
GGGGGGA278050.066.563656
TAGAGGG37000.066.481732
AAGGGAC19600.065.9418264
AGGGACT28500.065.797955
Produced by FastQC (version 0.11.5)