FastQCFastQC Report
Mon 27 Feb 2023
SRR3126827.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126827.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077894
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161970.7794911578742708No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51030.24558519346992674No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36950.17782427785055446No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG25080.12069913094700693No Hit
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG20840.10029385522071868No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58100.082.918961
AGGGATG27250.074.677775
ATAGGGC12550.073.772153
GTAGGGC8400.072.733423
AGTAGGG37200.072.390512
ACGGGAT5150.071.179894
CGTAGGG5700.070.907722
TAGGGCG3400.070.495474
CGAAGGG19650.069.598592
TAAGGGA18700.069.3645253
GAATAGG14050.069.314481
AGTAAGG14350.069.176821
GTACGGG4100.068.776062
AGGGTAC8700.068.604785
TAGGGCA10700.068.518964
TAGAGGG40450.067.968442
AGGGCGA11000.067.9325
ATAGCGG4100.067.701481
AGGGCAT19350.067.0344545
AAGAGGG70350.067.004942
Produced by FastQC (version 0.11.5)