FastQCFastQC Report
Mon 27 Feb 2023
SRR3126826.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126826.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314166
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197440.853179936097929No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT58130.25119200610500714No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41500.17933026412106998No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG28080.12133961003661795No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG24470.10574003766367666No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG23860.10310409884165612No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74150.083.9959641
AGGGATG32050.074.777375
CGTAGGG6600.073.589492
ACGGGAT6000.073.089374
AGTAGGG41100.072.165792
AGTAAGG14200.071.440711
AGGGTAC9200.070.4886555
ATAGGGC13800.070.3899463
TAGAGGG42650.070.206482
GAATAGG14600.070.129791
CGAAGGG21150.069.78412
ATAGAGG15750.069.203351
TGAAGGG41300.067.934352
GTAGGGC11550.067.7717363
AGGGCAT20900.067.678115
AGGGACT33900.067.369695
AAGGGTA14950.067.1832354
GGGCGAT10500.067.132056
GAGGGAT26650.067.060154
ACGGGTA1550.066.928874
Produced by FastQC (version 0.11.5)