FastQCFastQC Report
Mon 27 Feb 2023
SRR3126825.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126825.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2312884
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188700.8158645223884984No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT57490.2485641303238727No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39320.17000420254539356No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG28670.12395779468403949No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG24590.10631748068645035No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG23320.1008265005940635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72200.081.737911
CGTAGGG7100.078.817542
GTAGGGC11500.075.6040653
AGGGATG29700.074.530795
TAAGGGA19400.072.4337163
GGTAAGG14300.071.085481
CGAAGGG22050.070.3784262
ATAGGGC12250.070.2079543
AGTACGG2550.070.130451
AGTAAGG14750.068.597711
AGTAGGG37950.068.276952
TAGCGGG13450.068.178372
GTAAGGG36700.068.039752
TAGGGCA13000.067.965074
ATAGAGG15800.067.3154141
AAGAGGG77150.067.04882
ATGAGGG35900.066.936152
AAGGGTA14000.066.803064
AAGGGCG10500.066.6911544
GAGGGAT26300.066.653724
Produced by FastQC (version 0.11.5)