FastQCFastQC Report
Mon 27 Feb 2023
SRR3126824.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126824.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412742
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197460.8184049517105434No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT57780.23947856836744252No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA42230.1750290748036881No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG28190.11683802080786093No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG25790.10689083209062553No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG25150.10423824843269608No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC24930.10332642280028283Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74250.081.3106841
ACGGGAT5850.074.712834
AGGGATG33550.074.522465
CGTAGGG7550.072.829442
AGTAAGG15000.072.780351
ATAGGGC14050.070.913293
GGTAAGG16650.069.807191
CGAAGGG21650.069.029912
TAAGGGA20300.068.990343
AAGAGGG82000.068.718452
AGTAGGG41700.068.184782
AAGGGAT38900.068.018474
GTAGGGC11850.067.818143
AAGGGTA15250.067.798634
TAGGGCA11350.067.4931644
TAGGGCG4400.067.290844
AGAGGGC31750.066.905653
GAATAGG14650.066.489051
TAGCGGG12250.066.370962
AGGGAAT27850.066.318615
Produced by FastQC (version 0.11.5)