FastQCFastQC Report
Mon 27 Feb 2023
SRR3126822.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126822.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174864
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50020.4257514061201977No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41470.35297702542592163No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37360.31799425295183104No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG23700.20172547631044954No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT20170.17167944545070749No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG16800.14299527434664777No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG14490.12333342412398371No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA13690.11652412534557191No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC12810.10903389668931894Illumina Single End Adapter 2 (95% over 21bp)
GAAAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA12020.10230971414563728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG7100.084.730823
CGTTTTT27350.084.138771
AATGCGG2350.084.105821
AGGGATG24800.082.2484745
TAGCGGG4700.082.036882
GCTACGA7400.081.2957844
AGGGTAT8400.081.129455
ATTGCGG1750.080.6729351
CTACGAC7500.080.211855
ACGGGAT3300.079.7560964
AGGGTAC3900.079.5369955
TCCGCTA7600.079.257621
TAGGGTA4400.077.975824
GGTAAGG6600.077.7191
AAGGGCA15000.077.705234
CGTAGGG3650.077.295032
CGAAGGG8950.077.2302552
TACGACC7850.076.635526
AAGGGTA8750.076.272874
GGGTATC4650.075.8050466
Produced by FastQC (version 0.11.5)