Basic Statistics
Measure | Value |
---|---|
Filename | SRR3126821.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1230069 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 5214 | 0.4238786604653886 | No Hit |
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA | 4252 | 0.3456716655732321 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 4048 | 0.32908723006595564 | No Hit |
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG | 2367 | 0.19242822963589848 | No Hit |
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT | 2229 | 0.1812093467927409 | No Hit |
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG | 1867 | 0.15178010339257392 | No Hit |
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG | 1585 | 0.12885456019133887 | No Hit |
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA | 1431 | 0.11633493730839489 | No Hit |
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC | 1415 | 0.11503419726860852 | Illumina Single End Adapter 2 (95% over 21bp) |
GAAAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA | 1281 | 0.10414049943539753 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AATGCGG | 180 | 0.0 | 88.85713 | 1 |
CGTTTTT | 2585 | 0.0 | 84.98499 | 1 |
AGGGATG | 2535 | 0.0 | 83.79584 | 5 |
ATTGCGG | 135 | 0.0 | 83.63025 | 1 |
TAGCGGG | 610 | 0.0 | 81.658745 | 2 |
CGTAGGG | 360 | 0.0 | 80.931175 | 2 |
CGCTACG | 790 | 0.0 | 80.89813 | 3 |
CTAGCGG | 105 | 0.0 | 80.64345 | 1 |
GCTACGA | 800 | 0.0 | 80.477585 | 4 |
CGAAGGG | 1065 | 0.0 | 79.864845 | 2 |
GAATAGG | 845 | 0.0 | 79.05285 | 1 |
GGTAAGG | 760 | 0.0 | 78.60967 | 1 |
CGAGGGA | 535 | 0.0 | 78.1741 | 3 |
TCCGCTA | 825 | 0.0 | 78.118256 | 1 |
ATAGCGG | 235 | 0.0 | 78.069725 | 1 |
AAGGGCA | 1845 | 0.0 | 77.94161 | 4 |
AGGGTAC | 420 | 0.0 | 77.2079 | 5 |
ATAGAGG | 720 | 0.0 | 77.096634 | 1 |
GGAATGC | 985 | 0.0 | 76.81599 | 7 |
AGGGCAT | 1110 | 0.0 | 76.63331 | 5 |