FastQCFastQC Report
Mon 27 Feb 2023
SRR3126821.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126821.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230069
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52140.4238786604653886No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA42520.3456716655732321No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40480.32908723006595564No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG23670.19242822963589848No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT22290.1812093467927409No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG18670.15178010339257392No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG15850.12885456019133887No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA14310.11633493730839489No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC14150.11503419726860852Illumina Single End Adapter 2 (95% over 21bp)
GAAAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA12810.10414049943539753No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGG1800.088.857131
CGTTTTT25850.084.984991
AGGGATG25350.083.795845
ATTGCGG1350.083.630251
TAGCGGG6100.081.6587452
CGTAGGG3600.080.9311752
CGCTACG7900.080.898133
CTAGCGG1050.080.643451
GCTACGA8000.080.4775854
CGAAGGG10650.079.8648452
GAATAGG8450.079.052851
GGTAAGG7600.078.609671
CGAGGGA5350.078.17413
TCCGCTA8250.078.1182561
ATAGCGG2350.078.0697251
AAGGGCA18450.077.941614
AGGGTAC4200.077.20795
ATAGAGG7200.077.0966341
GGAATGC9850.076.815997
AGGGCAT11100.076.633315