FastQCFastQC Report
Mon 27 Feb 2023
SRR3126820.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126820.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401100
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170510.7101328557744366No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT55540.23131064928574402No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA45510.18953812835783598No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33510.13956103452584231No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG31050.12931573029028362No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG29380.12236058473199783No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC25570.10649285744033984No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59200.083.517111
AGGGCAT23000.077.844785
CGTAGGG6300.077.831672
TAGGGCG3900.077.3695144
TAGGGCA13500.074.3875664
TAACGGG7500.074.179572
AGAGGGC29050.073.844773
AGGGATG36800.073.809525
ACGGGAT5750.073.795384
GGGCGAT10450.073.299956
GTAGGGC12400.072.241453
AAGGGTA16550.072.073744
TAGGGTC7150.071.874534
CTAACGG1650.071.4812241
AAGGGCA35650.071.414894
AGTAAGG15550.070.993961
AGTAGGG39950.070.574532
TGTAGGG16650.070.5096742
AACGAGG9050.070.375431
TAGAGGG39850.070.278372
Produced by FastQC (version 0.11.5)