FastQCFastQC Report
Mon 27 Feb 2023
SRR3126819.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126819.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2403086
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163450.6801670851563365No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT55320.2302039960284401No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA43540.18118369463265152No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33970.14135990139345825No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG30260.12592141937492043No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG29930.12454818512529306No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC25530.10623839513026166No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57850.082.956391
AGGGCAT22700.080.330355
GTAGGGT7950.075.668773
ACGGGTA2500.075.195844
AGGGATG38600.073.9052355
AGGGTAC9650.073.539975
AGTAAGG16400.072.582121
CGTAGGG7000.072.540472
CGAAGGG23050.072.412252
TAGGGTC5600.072.1745764
AAGGGCA35100.071.9024
ATAGCGG6700.071.6272661
AGAGGGC32300.070.7143553
TAAGGGA22000.070.709723
GTAGGGC10950.070.3887943
TAGCGGG12700.070.340342
AAGGGTA15850.069.97724
GAGGGCA23750.069.8529744
AGGGCAC15500.069.7380755
ACGGGAT5500.069.214354
Produced by FastQC (version 0.11.5)