FastQCFastQC Report
Mon 27 Feb 2023
SRR3126818.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126818.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2546235
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172230.6764104648628269No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT58460.22959389058747523No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA45240.17767409528185735No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35100.13785059116695828No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG31850.12508664754038806No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG31690.12445826877723384No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC27470.10788477889904113No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60900.085.0607761
ATAGCGG6600.076.990851
AGGGCAT23650.075.5136265
ACGGGAT5200.075.014924
AGTAAGG18750.074.0252761
AATGCGG5650.072.4486161
GAATAGG14650.072.261041
AGGGATG38950.072.155035
CGTAGGG6900.071.517632
TAGCGGG13900.071.341232
CGGGATA4300.071.0424355
AGAGGGC34350.070.87223
TAAGGGA22600.070.495953
TAGGGTA9150.070.367544
CGAAGGG22500.069.973762
GTAAGGG41850.069.513332
AGTAGGG44700.069.181712
AAGGGTA18900.069.128314
ATAAGGG36350.069.041432
GGTAAGG15950.069.0262761
Produced by FastQC (version 0.11.5)