FastQCFastQC Report
Mon 27 Feb 2023
SRR3126817.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126817.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334181
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283111.2128879465645552No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53300.22834561672809436No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA29570.12668254946810037No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT103300.087.659411
CGTAGGG7050.078.234892
TAGGGCA13350.074.859864
GTAGGGC11400.073.605383
GTAGGGT8500.072.651863
AGGGATG30750.071.8268365
TAGGGCG4800.071.692884
ATAGGGC15450.071.397293
AGTAAGG17050.071.12671
AGTAGGG35350.070.945682
TGTAGGG16450.069.637642
ACGGGTA1900.069.4702454
TAGAGGG39800.069.290952
TAAGGGA20950.068.404393
TAGCGGG10400.067.99262
AGTACGG3500.067.410231
AGGGAAT30500.066.868865
GAATAGG14450.066.617181
AGAGGGC33300.066.534673
ACGGGAT6100.066.460414
Produced by FastQC (version 0.11.5)