FastQCFastQC Report
Mon 27 Feb 2023
SRR3126816.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126816.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333511
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273971.1740677459844842No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51860.22224022085175513No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA29000.12427625153684727No Hit
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT24180.10362068145382645TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT103100.086.919241
ATAGCGG4900.079.7241061
CGTAGGG8250.075.2378542
GTAGGGT8650.072.803763
AAGGGTA14450.072.202174
ATAGGGC14600.072.104153
AGTAAGG15450.071.589121
TAGGGCA11950.071.576194
TAGCGGG11850.071.4283452
AGGGATG31500.071.3152545
AGGGCAT18900.070.3703465
AGAGGGC33700.069.169853
GGTAAGG14850.069.0935751
AGGGTAT12600.069.002735
TAAGGGA21050.068.987663
TATAGGG18150.068.138962
TAGAGGG39950.068.0342252
ATAAGGG35150.067.960222
GTACGGG4650.067.7545242
AGTAGGG33800.067.474782
Produced by FastQC (version 0.11.5)