FastQCFastQC Report
Mon 27 Feb 2023
SRR3126815.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126815.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424897
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285751.1784005671168714No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53660.22128774954152694No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA29530.12177836831832445No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG25000.10309716247741657TruSeq Adapter, Index 16 (100% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT24540.10120017468783209TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT107900.086.920361
ACGGGTA2200.074.766584
ACGGGAT7400.073.669624
CGTAGGG8750.073.582522
ATAGGGC14350.072.704833
GTAGGGC11600.072.114753
GGTAAGG14350.071.81711
TAGCGGG11800.071.689022
TAAGAGG18100.071.497321
TAGGGCG4300.071.0406654
AGGGATG28700.070.576085
AGTAAGG16000.070.2932361
AGGGCAT20100.070.1434865
TAGAGGG42350.070.0225752
TAGGGCA11450.069.365474
AGGGTAC9850.069.182125
AGAGGGC35000.069.151453
TAAGGGA22650.068.678683
TAAACGG2950.068.593361
TATAGGG22000.068.571632
Produced by FastQC (version 0.11.5)