FastQCFastQC Report
Mon 27 Feb 2023
SRR3126814.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126814.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421620
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT452503.1829884216597963No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTT39450.277500316540285No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCT37460.26350220171353805No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT34700.24408773089855235No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32840.23100406578410543No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG23520.16544505564074788No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC19770.13906669855516945No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTC18210.12809330200756883No Hit
CGTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC15500.10903054262039083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT138300.090.608051
ACGGGTC1750.072.743694
TAGGGCA5800.072.348854
CGGGAAT2300.071.5107965
ACGGGAT2650.071.167834
ATAGCGG3250.071.133411
ATAGGGC8050.070.869463
TAGCGGG7200.070.068192
ATGTAGG4400.069.6982041
CGTAGGG3250.069.634992
GTACGGG2850.069.482282
ATAGGGA10750.069.297613
AGGGACT19400.068.7935945
ATAGAGG10100.068.668381
GGGCGAT6450.068.485646
AGGGATG18400.068.4460455
GAGGGTA6350.068.3099064
AGTAGGG21950.068.091732
GCATAGG3750.067.939661
ACGGGTA1400.067.355274