FastQCFastQC Report
Mon 27 Feb 2023
SRR3126811.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126811.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237272
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195070.8719100761999434No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT49160.21973188776331173No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39180.17512399028817238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70450.084.433271
ACGGGAT5700.081.876154
CGTAGGG7700.075.306242
TAGGGCG4000.074.246784
AGTAAGG12600.073.7522351
GGTAAGG11150.073.1898351
AGTAGGG37000.072.6255952
CGAAGGG20650.070.543172
ATAGAGG18050.069.777211
TAGACGG3250.069.6686551
TAGGGCA11600.069.4920654
AAGGGTA15100.068.994174
ATGAGGG35900.068.8102952
TAGAGGG41800.068.571532
AGGGATG26100.068.239345
TAAGGGA18350.067.821113
ATAGGGA19900.067.513233
AGGGCGA11500.067.425195
GTAGGGC11200.067.3440253
AAGAGGG77650.067.0868452
Produced by FastQC (version 0.11.5)