FastQCFastQC Report
Mon 27 Feb 2023
SRR3126810.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126810.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238503
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188110.8403383868594324No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT48270.21563518119028657No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41730.18641922749265916No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67100.083.518691
AAGGGTA14800.074.6253054
CGTAGGG7800.073.5426562
GGTAAGG11950.072.450991
TAGGGTC5450.071.575044
AGTAGGG39250.071.0377352
TAGGGCA11950.070.792074
ATAGGGC13900.069.989933
GTAGGGT8850.069.567763
ATAGAGG17250.069.557751
ATGAGGG34750.069.412332
AGGGCAT18350.069.152475
ACGGGAT6050.069.137654
AGGGTAC8300.068.515265
AGTAAGG14200.068.26111
TAAGAGG17400.068.146841
TAAGGGA18250.067.728723
ATAGGGA18900.067.637433
TATAGGG18800.067.027182
GAATAGG15200.066.865891
Produced by FastQC (version 0.11.5)