FastQCFastQC Report
Mon 27 Feb 2023
SRR3126809.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126809.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2370526
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198450.8371559729781491No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT49580.2091518928710337No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA40600.1712700050537307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75550.083.477421
ATAGAGG17100.072.386611
TAGGGCA11900.072.27054
GGTAAGG12250.072.230461
AGGGCAT21100.071.495635
ATAGGGC12650.071.329253
AGTAGGG40650.070.637862
TAAGGGA18500.070.620383
AAGGGTA15250.070.57044
ACGGGTA2800.070.4933554
AGTAAGG16050.070.3776551
ACGGGAT7100.069.5004964
GAGGGAT27000.068.7527854
CGTAGGG7600.068.638272
AGGGATG29400.067.7759255
GTAAGGG36650.067.576172
TAACGGG8350.067.538552
CGAAGGG24350.067.35712
GTACGGG6350.067.3479842
TAGAGGG45250.067.2997362
Produced by FastQC (version 0.11.5)