FastQCFastQC Report
Mon 27 Feb 2023
SRR3126808.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126808.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433958
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388091.594481088005627No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT46160.18964994465804258No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT39000.16023283885753165No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT36040.14807157724167794No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG31960.13130875717658233TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT30110.12370796866667379No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC27670.11368314490225385TruSeq Adapter, Index 20 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26660.10953352522927676No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG24670.10135754191321297TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT127900.088.32921
TAGGGCA12500.076.5494464
ACGGGTA2800.075.754864
ATAGCGG5300.075.6506041
ACGGGAT5850.073.323234
ATAGGGC15300.072.7070543
AGTAGGG36150.072.238112
AGGGATG32100.072.0267265
AGGGCAT23000.071.9197855
AGAGGGC33950.070.808663
CGTAGGG6200.070.7059862
GTAGGGT8650.069.750913
AGGGTAC10400.069.1345
GTAGGGC10500.069.1333163
CGAGGGA14150.068.955663
CGAAGGG21600.068.5237052
AGGGACT32750.068.3013465
TAGGGCG4500.068.0858464
TAAGGGA20200.067.904353
AAGGGCA35300.067.700094
Produced by FastQC (version 0.11.5)