FastQCFastQC Report
Mon 27 Feb 2023
SRR3126807.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126807.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435480
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370011.5192487723159296No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT46260.18994202374891192No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT40010.1642797313055332No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT37210.15278302429089954No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG32240.13237636933992478TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT30740.1262174191535139No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC29740.1221114523625733TruSeq Adapter, Index 20 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27120.11135381937030893No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG24560.10084254438550101TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT129400.087.902041
GTAGGGC12500.074.063823
TAGGGCA13450.073.025394
ACGGGAT6050.072.239954
CGTAGGG6250.072.224062
GTACGGG4950.072.1936652
AGGGCAT19750.072.0983665
AGTAGGG38950.071.949782
ATAGGGA22000.070.064993
ATAGCGG5400.069.719261
AGAGGGC37350.069.5799943
ACGGGTC2250.068.925724
AGGGATG34600.068.726515
TAAGGGA21850.068.610263
ATAAGGG34550.068.455862
ATAGAGG17400.067.615661
TAGACGG3550.067.608051
GGTAAGG16950.067.189631
AGGGTAC9600.067.0655065
ATAGGGC15100.066.601913
Produced by FastQC (version 0.11.5)