FastQCFastQC Report
Mon 27 Feb 2023
SRR3126806.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126806.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2560215
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT384841.5031550084660859No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT48460.18928097835533345No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT40740.15912726079645656No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT39110.15276060799581287No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG35140.13725409780038006TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT33080.12920789855539475No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC32100.12538009503108138TruSeq Adapter, Index 20 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28820.11256867099052228No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG26030.10167114871211988TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT133150.087.763991
ACGGGTA2500.078.954884
ATAGCGG5650.074.134841
AGGGCAT22200.073.45925
ACGGGAT6200.072.769484
AGGGATG34700.072.052975
TAGGGCG3600.071.80094
ATAGGGC15300.071.261963
TAGGGCA12650.070.959824
GTAGGGC10950.069.957573
AGTAGGG42150.069.7971952
AAGGGCA37250.069.769974
CGTAGGG7400.069.223892
AGGGACT35550.068.743785
AGGGTAC10400.068.687855
AGGGAAT28550.068.643535
GTACGGG5350.068.517582
TGTAGGG17800.068.3818742
GGTAAGG14500.068.16041
AGAGGGC37950.068.110193
Produced by FastQC (version 0.11.5)