FastQCFastQC Report
Mon 27 Feb 2023
SRR3126805.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126805.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1579667
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102930.6515930256186906No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC80930.5123231668446577No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA48260.3055074265652191No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG29690.1879510048636833No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29200.18484908528189803No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT26280.16636417675370824No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC25400.1607933824027469Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG24670.15617215527069947No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG20510.12983749106615508No Hit
AAAGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT19040.12053173232079925No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTGC17520.11090945116913882No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC16860.10673135540591784No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT37400.085.902151
ACGGGAT6750.085.202734
AGGGATG34850.084.007815
TACGGGT1350.083.805963
TAGGGTC4000.081.317974
CGCTACG13450.081.313583
GCTACGA13550.080.365584
AGGGCGA6100.080.119445
TCCGCTA14050.079.915151
ACGGGTC1950.079.776834
AGGGCGT1950.079.526845
TAGGGCG2550.079.4924244
AGGGATT16450.079.416975
CGACCAA13250.079.0903248
AGTAAGG8650.079.0825041
TAAGGGA16500.078.5680853
CGAAGGG13750.078.510952
AGGGTAC6050.077.67465
TAGCGGG8050.077.2992862
GGATGGC23950.076.915697
Produced by FastQC (version 0.11.5)