FastQCFastQC Report
Mon 27 Feb 2023
SRR3126804.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126804.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589238
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97350.6125577163395288No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC79350.49929588897320604No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA46850.2947953673395678No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG30540.19216756709819424No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28920.18197400263522517No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC25950.16328580111978194Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG25470.16026548572334665No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT25260.15894409773740623No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG19980.12572062837661824No Hit
AAAGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT19600.12332954535444031No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTGC16790.10564811563780882No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC16620.10457842060157133No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTC3450.085.820944
ACGGGAT7150.084.792124
CTAGCGG1500.084.70421
AGGGATG35150.084.367635
CGTTTTT37050.083.573791
TAGCGGG8000.081.1058652
ATAGCGG3900.080.843031
AGGGCGT1700.080.171675
AGGGATT15900.079.510965
CGACCAA14000.079.223828
CGCTACG14200.079.100893
AGCGGGT4300.078.6929553
ACGGGTC2100.078.328644
AAGGGTA10050.078.094824
AGGGCGA7650.078.021475
GGATGGC27000.077.9805157
GAGGGAT25600.077.65554
AGGGAAT26300.077.554285
TCACGGG1700.077.441242
TAAGGGA14850.077.220973