Basic Statistics
Measure | Value |
---|---|
Filename | SRR3126802.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2463262 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 14986 | 0.6083802697398815 | No Hit |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 5307 | 0.21544602238819907 | No Hit |
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA | 5067 | 0.20570284443960896 | No Hit |
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG | 3376 | 0.13705403647683437 | Illumina PCR Primer Index 7 (95% over 22bp) |
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT | 2859 | 0.11606560731257984 | No Hit |
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC | 2794 | 0.11342682995150333 | TruSeq Adapter, Index 23 (96% over 25bp) |
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG | 2718 | 0.11034149026778312 | Illumina PCR Primer Index 7 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 5795 | 0.0 | 81.79465 | 1 |
CGAAGGG | 2470 | 0.0 | 75.373825 | 2 |
GGGCGAT | 1020 | 0.0 | 73.25499 | 6 |
AGTAAGG | 1725 | 0.0 | 71.36145 | 1 |
AGGGCGA | 1140 | 0.0 | 71.31511 | 5 |
CGTAGGG | 695 | 0.0 | 71.20737 | 2 |
AGGGATG | 3170 | 0.0 | 71.15775 | 5 |
TAGAGGG | 4220 | 0.0 | 71.0338 | 2 |
TAGCGGG | 1255 | 0.0 | 70.98041 | 2 |
AGTAGGG | 4020 | 0.0 | 70.69874 | 2 |
AAGGGTA | 1635 | 0.0 | 70.33411 | 4 |
AATGCGG | 620 | 0.0 | 69.98555 | 1 |
TAGGGCG | 535 | 0.0 | 69.59326 | 4 |
AGGGCAT | 2385 | 0.0 | 69.35768 | 5 |
GTAAAGG | 2370 | 0.0 | 69.25373 | 1 |
ACGGGTA | 225 | 0.0 | 69.12343 | 4 |
TAGGGCA | 1345 | 0.0 | 69.02999 | 4 |
GGTAAGG | 1515 | 0.0 | 68.80055 | 1 |
ACGGGAT | 555 | 0.0 | 68.78377 | 4 |
GTAAGGG | 3910 | 0.0 | 68.70976 | 2 |