FastQCFastQC Report
Mon 27 Feb 2023
SRR3126802.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126802.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463262
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149860.6083802697398815No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53070.21544602238819907No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA50670.20570284443960896No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG33760.13705403647683437Illumina PCR Primer Index 7 (95% over 22bp)
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT28590.11606560731257984No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC27940.11342682995150333TruSeq Adapter, Index 23 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG27180.11034149026778312Illumina PCR Primer Index 7 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57950.081.794651
CGAAGGG24700.075.3738252
GGGCGAT10200.073.254996
AGTAAGG17250.071.361451
AGGGCGA11400.071.315115
CGTAGGG6950.071.207372
AGGGATG31700.071.157755
TAGAGGG42200.071.03382
TAGCGGG12550.070.980412
AGTAGGG40200.070.698742
AAGGGTA16350.070.334114
AATGCGG6200.069.985551
TAGGGCG5350.069.593264
AGGGCAT23850.069.357685
GTAAAGG23700.069.253731
ACGGGTA2250.069.123434
TAGGGCA13450.069.029994
GGTAAGG15150.068.800551
ACGGGAT5550.068.783774
GTAAGGG39100.068.709762