FastQCFastQC Report
Mon 27 Feb 2023
SRR3126801.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126801.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2468282
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142330.5766358949261065No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51830.20998411040553713No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA49540.20070640226683986No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG32960.13353417478229795Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC29170.11817936524270728TruSeq Adapter, Index 23 (96% over 25bp)
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT27950.11323665610331397No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG27840.11279100200058177Illumina PCR Primer Index 7 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50250.083.123071
AGTAAGG16500.076.400381
CGTAGGG6150.075.672952
CGAAGGG26850.074.5840452
AGGGCAT22750.072.303895
AGTAGGG40000.072.1587452
TAGGGCG4850.071.7075654
TAGGGAC12050.071.373844
AGGGATG31350.070.7586445
TAGGGCA14200.070.165324
AGAGGGC35250.069.996323
GGTAAGG15400.069.945441
TAGCGGG12400.069.376122
CGAGGGA14000.069.15353
GAATAGG15450.069.110191
TAGAGGG45900.068.925982
ATAGCGG4850.068.8590851
GTAAGGG41050.068.366252
GGGGGGA339000.067.9315266
AGCGGGG33100.067.8695453
Produced by FastQC (version 0.11.5)