FastQCFastQC Report
Mon 27 Feb 2023
SRR3126799.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126799.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2278683
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131610.5775704650449404No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT64150.28152226527340574No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA52840.23188833198825812No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38080.16711407422620872No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT32270.14161689010713643No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG32040.14060753514200966No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG26930.11818230091680151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG4300.085.560981
CGTTTTT49600.081.688541
CGTAGGG6050.078.695132
AGGGCAT19800.075.711465
TAGCGGG11450.074.928632
GGTAAGG15050.074.278211
ACGGGTA2700.073.324364
TAGGGCG4400.072.848234
GAATAGG14650.071.476781
CGAAGGG20400.071.401972
TAAGGGA19100.071.322623
AGGGATG32000.071.07745
AGTAGGG36250.070.611222
TAACGGG7700.070.402652
ATAGGGC13900.070.196953
TAGAGGG42400.070.1528552
AACGAGG8100.069.878861
AGAGGGC30300.069.8500063
TAGGGCA13300.069.8195954
TATAGGG18300.069.8071752
Produced by FastQC (version 0.11.5)