FastQCFastQC Report
Mon 27 Feb 2023
SRR3126797.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126797.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2409353
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136270.5655875249496441No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT66230.27488707549288127No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA54200.2249566584888142No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40260.16709880204353617No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT32270.13393637212977924No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG32170.13352132294437552No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG29430.12214897526431369No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50100.081.336521
CGTAGGG7550.076.5636142
GGTAAGG14550.076.3228151
TACGGGT2350.073.9942863
TAGCGGG13100.073.543932
GTAGGGT10950.073.391593
AGGGCAT20750.073.1557855
TAACGGG7400.071.129642
GTACGGG5600.070.494552
AGTAGGG41850.070.410332
AGGGATG34000.069.388755
TAGAGGG45000.069.136882
AGTAAGG14450.069.0356451
ATAGCGG5400.068.839661
TATAGGG19400.068.7988052
GAATAGG15600.068.470991
ATAGAGG16650.068.39221
GAGGGAT29150.068.358364
TAAGGGA18150.068.358353
AGAAGGG86400.068.210012
Produced by FastQC (version 0.11.5)