FastQCFastQC Report
Mon 27 Feb 2023
SRR3126796.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126796.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173596
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56710.48321568921502794No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA43050.3668212911427783No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33220.2830616327935678No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24040.20484050729552586No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG23670.20168780398024533No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG23040.19631968752449735No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC21630.1843053316473471No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT16540.1409343590128119No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA12380.1054877487653332No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGT156.9062307E-493.986377
CGTTTTT28050.085.2737961
CGAAGGG9650.083.54342
AGGGCAT11350.083.221415
TAGCGGG4950.082.862392
AAGGGCA12850.082.551094
AGGGATG19250.081.292115
GGTAAGG5700.081.113241
ATCGGGT353.7825885E-880.821453
GTAGGGC4950.080.0050663
CGTAGGG2300.079.942952
CACGGGT650.079.785273
TAACGGG3750.079.205022
AAGGGAC8700.078.57644
AACGAGG3550.078.408691
GTAAAGG8200.078.246621
TCCGCTA6700.078.160751
AGGGTAC4100.077.939915
ACGGGTC1150.077.893134
ACGGGAT3650.077.5000154