FastQCFastQC Report
Mon 27 Feb 2023
SRR3126794.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126794.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206451
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56480.46814997045051976No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44020.3648718431167118No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32530.2696338268193238No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG25670.21277283536587896No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG24900.20639047918232897No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24280.20125143913843163No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC22800.1889840532271928No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT16330.13535568373684467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGT301.326589E-893.9931643
CTAGCGG1250.086.592131
CGTTTTT31400.085.42911
GGGCGAT5750.084.185186
CGAAGGG9400.082.4942
TAGCGGG6400.081.50972
AGGGATG19100.081.4443365
AGGGCAT9350.080.924595
TAAGGGA12200.080.895753
AGGGCGA6350.080.67135
GGTAAGG7300.080.583811
AGGGTCA4750.080.141545
AAGGGAC9800.079.6064454
GTAGGGT3250.079.532683
GTAGGGC4750.079.152143
GGATGGC11250.078.536517
GCATAGG3500.077.986711
AGTAAGG8750.077.448871
CGGGTCA850.077.4061365
AGAGGGC14250.077.173343
Produced by FastQC (version 0.11.5)