FastQCFastQC Report
Mon 27 Feb 2023
SRR3126793.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126793.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2300884
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161680.7026864457312929No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT50990.22161047666896722No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA37400.1625462213653535No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG25020.1087408144000306No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60250.085.594471
CGTAGGG7400.079.00772
ACGGGTA2650.072.943914
ATAGCGG5000.072.661331
AGGGCAT21950.070.864145
AGGGATG31350.070.751815
TAGAGGG41750.070.35762
CGAAGGG22700.069.997662
AGAGGGC28500.069.644743
GAATAGG15700.069.4216461
GGTAAGG13800.069.40641
TAGCGGG12250.069.281282
GAGGGAT25700.068.610344
ATAGGGC13300.068.415853
GTAGGGC10350.068.3285143
AGTAGGG38250.068.166842
AGGGCAC16100.067.716615
TGTAGGG18900.067.3568042
AGGGCGA11000.067.0718155
TAAGGGA23000.067.0302663
Produced by FastQC (version 0.11.5)