FastQCFastQC Report
Mon 27 Feb 2023
SRR3126792.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126792.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2300637
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155840.6773776132436364No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51060.22193853267595018No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35950.15626107030357245No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24880.10814396186795223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59100.083.60241
CGTAGGG7250.073.935522
TAGGGCA13950.071.421814
TAGCGGG13250.070.974182
AGGGCAT21600.070.2777565
AGTAGGG38700.070.105352
AGGGATG30550.069.841545
ATAGCGG5500.069.300961
GTAGGGC11800.069.30053
AGGGTAC9400.068.995445
CGAAGGG21850.068.217232
TATAGGG19150.068.013842
TAAGGGA21750.067.848393
AATGCGG6800.067.81631
AGACGGG15000.067.3959052
GGTAAGG12750.067.170431
ACGGGAT6600.066.934974
AGGGACT35550.066.62855
GAATAGG15050.066.5978241
ATAGGGC13850.066.508243
Produced by FastQC (version 0.11.5)