FastQCFastQC Report
Mon 27 Feb 2023
SRR3126791.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126791.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418469
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162490.6718713367837256No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53010.21918825504895867No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39010.16130039293453835No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG24230.10018734993088602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61200.084.563661
CGTAGGG7950.074.482082
GTAGGGC11400.072.965373
AGTACGG2650.072.7914661
TAGGGCA13700.072.721644
ATAGCGG5450.072.514571
ACGGGAT6450.071.402724
ATAGGGC14650.070.572163
AGGGCAT22650.070.128875
AGGGATG34250.069.9774255
TAAGGGA22800.069.66753
ACGGGTA1900.069.2552644
AGTAGGG41450.068.479542
CGAAGGG20950.068.192712
GGGCGAT12400.067.839046
TATAGGG20350.067.663052
GGTAAGG14250.067.022971
TAGCGGG13550.066.937022
TAGAGGG44650.066.834492
GAATAGG16700.065.9237441
Produced by FastQC (version 0.11.5)